病理型
生物
野油菜黄单胞菌
爆发
黄单胞菌
微生物学
病毒学
遗传学
细菌
假单胞菌科
假单胞菌
作者
Verónica Román-Reyna,Rebecca D. Curland,Yesenia Vélez-Negrón,Kristi E. Ledman,Diego E. Gutierrez-Castillo,Jonathan Beutler,Jules Butchacas,Gurcharn S. Brar,R. H. Roberts,Ruth Dill‐Macky,Jonathan M. Jacobs
出处
期刊:Phytopathology
[American Phytopathological Society]
日期:2022-10-13
卷期号:113 (11): 2110-2118
被引量:7
标识
DOI:10.1094/phyto-07-22-0262-sa
摘要
Bacterial leaf streak, bacterial blight, and black chaff caused by Xanthomonas translucens pathovars are major diseases affecting small grains. Xanthomonas translucens pv. translucens and X. translucens pv. undulosa are seedborne pathogens that cause similar symptoms on barley, but only X. translucens pv. undulosa causes bacterial leaf streak of wheat. Recent outbreaks of X. translucens have been a concern for wheat and barley growers in the Northern Great Plains; however, there are limited diagnostic tools for pathovar differentiation. We developed a multiplex PCR based on whole-genome differences to distinguish X. translucens pv. translucens and X. translucens pv. undulosa. We validated the primers across different Xanthomonas and non-Xanthomonas strains. To our knowledge, this is the first multiplex PCR to distinguish X. translucens pv. translucens and X. translucens pv. undulosa. These molecular tools will support disease management strategies enabling detection and pathovar incidence analysis of X. translucens. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
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