小泡
膜
相(物质)
生物物理学
化学物理
脂质双层
相变
传单(植物学)
结晶学
化学
纳米尺度
细胞质
脂质微区
材料科学
纳米技术
凝聚态物理
物理
生物
生物化学
古生物学
有机化学
作者
Gerald W. Feigenson,Thais A. Enoki
标识
DOI:10.1016/j.bpj.2022.11.020
摘要
Unfavorable lipid-lipid pairwise interactions between HiTm and LowTm lipids drive liquid-disordered (Ld) + liquid-ordered (Lo) phase separation. Large size of phase domains is opposed by lipid dipole repulsions, which are more significant compared with the pairwise interactions for naturally abundant LowTm lipids such as palmitoyl oleoyl phosphatidylcholine. During the nano-to-macro domain size transition, no lipid phase transition occurs, and measured properties of Ld + Lo nanodomains are found to be essentially the same as those of macrodomains. Use of macrodomains in mixtures to model cell plasma membranes (PM) is helpful, enabling study by optical microscopy. Use of asymmetric giant unilamellar vesicles to model a PM reveals that ordered phase domains in one leaflet induce ordered domains in an otherwise uniform phase in the apposing leaflet that models a cytoplasmic leaflet. Because macro and nano phase properties are so similar, we conclude that a cell PM that has nano-scale Ld + Lo phase domains in the exoplasmic leaflet is likely to induce nano-scale ordered domains in the cytoplasmic leaflet.
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