Regulatory network of ginsenoside biosynthesis under Ro stress in the hairy roots of Panax ginseng revealed by RNA sequencing

人参 茉莉酸甲酯 水杨酸 人参皂甙 五加科 生物 玉米素 下调和上调 传统医学 基因 生物化学 生长素 医学 细胞分裂素 病理 替代医学
作者
Xiangru Meng,Tao Zhang,Changbao Chen,Qiong Li,Jingwan Liu
出处
期刊:Frontiers in Bioengineering and Biotechnology [Frontiers Media]
卷期号:10 被引量:6
标识
DOI:10.3389/fbioe.2022.1006386
摘要

P. ginseng C.A. Meyer is a valuable Chinese herbal medicine that belongs to the Araliaceae family. Major obstacles to the continuous cropping of ginseng have severely restricted the sustainable development of the ginseng industry. The allelopathic effects of triterpenoid saponins play an important role in disorders related to continuous cropping; however, the mechanisms underlying the allelopathic autotoxicity of triterpenoid ginsenosides remain unknown. In this study, we performed mRNA and miRNA sequencing analyses to identify candidate genes and miRNAs that respond differentially to ginsenoside Ro stress in ginseng and their targets. The growth of the ginseng hairy roots was significantly inhibited under Ro stress (0.5 mg/L, Ro-0.5). The inhibition of root growth and injury to root-tip cells promoted the accumulation of the endogenous hormones indole-3-acetic acid and salicylic acid and inhibited the accumulation of abscisic acid and jasmonate acid. The accumulation of ginsenosides, except Rg3, was significantly inhibited under Ro-0.5 stress. An mRNA analysis of the Ro-0.5 and control groups showed that differentially expressed genes were mostly concentrated in the hormone signal transduction pathway. ARF7 and EFM were upregulated, whereas XTH23 and ZOX1 were downregulated. These genes represent important potential candidates for hormone-responsive continuous cropping diseases. In total, 74 differentially expressed miRNAs were identified based on the miRNA sequencing analysis, of which 22 were upregulated and 52 were downregulated. The target genes of ptc-miR156k_L + 1, mtr-miR156b-5p, gma-miR156a_R + 1, and mtr-miR156e all belonged to TRINITY_DN14567_c0_g4, which is a gene in the plant hormone signal transduction pathway. These four miRNAs were all negatively correlated with mRNA, indicating their likely involvement in the response of ginseng to continuous cropping disorders and the regulation of ginsenoside synthesis. Our findings provide useful insights for removing the barriers to continuous ginseng cropping and have important implications in the genetic engineering of plant stress responses.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
CipherSage应助CC采纳,获得10
3秒前
qinghong完成签到,获得积分10
4秒前
沉静智宸完成签到 ,获得积分10
5秒前
5秒前
花蝴蝶完成签到 ,获得积分10
7秒前
xuli-888完成签到,获得积分10
7秒前
car子完成签到 ,获得积分10
9秒前
Jes发布了新的文献求助30
9秒前
畅快的长颈鹿完成签到,获得积分10
15秒前
15秒前
克泷完成签到 ,获得积分10
19秒前
共享精神应助阿司匹林采纳,获得10
19秒前
坚强的蚂蚁完成签到,获得积分10
20秒前
Vivian完成签到,获得积分10
21秒前
脑洞疼应助雪山飞龙采纳,获得10
24秒前
健康的修洁完成签到 ,获得积分10
25秒前
25秒前
28秒前
CCC完成签到 ,获得积分10
35秒前
嗨Honey完成签到 ,获得积分10
37秒前
超chao完成签到,获得积分10
38秒前
糊涂的麦片完成签到,获得积分10
39秒前
Wind发布了新的文献求助20
40秒前
sylens完成签到,获得积分10
40秒前
科研通AI5应助张先生采纳,获得10
41秒前
昔年完成签到 ,获得积分10
42秒前
小全完成签到,获得积分10
43秒前
45秒前
香菜大王完成签到 ,获得积分10
45秒前
48秒前
Jro完成签到,获得积分10
48秒前
研友_Y59785应助云栖采纳,获得10
49秒前
北风应助nnnn采纳,获得10
51秒前
51秒前
pluto应助可耐的无剑采纳,获得50
52秒前
sylens发布了新的文献求助10
54秒前
雪山飞龙发布了新的文献求助10
55秒前
xiaohanzai88完成签到,获得积分10
59秒前
缥缈的青旋完成签到,获得积分10
1分钟前
高分求助中
【此为提示信息,请勿应助】请按要求发布求助,避免被关 20000
Continuum Thermodynamics and Material Modelling 2000
Encyclopedia of Geology (2nd Edition) 2000
105th Edition CRC Handbook of Chemistry and Physics 1600
Maneuvering of a Damaged Navy Combatant 650
Периодизация спортивной тренировки. Общая теория и её практическое применение 310
Mixing the elements of mass customisation 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3779565
求助须知:如何正确求助?哪些是违规求助? 3325025
关于积分的说明 10221059
捐赠科研通 3040157
什么是DOI,文献DOI怎么找? 1668640
邀请新用户注册赠送积分活动 798728
科研通“疑难数据库(出版商)”最低求助积分说明 758522