Conformation-induced ratiometric electrochemical aptasensor for the detection of aflatoxin B1 in urban wetland environments

Monitoring waterborne pathogens with high specificity and sensitivity is critical for public health and environmental safety. Herein, we developed a ratiometric electrochemical biosensing platform utilizing aptamer recognition-triggered, enzyme-free catalytic hairpin assembly-assisted signal amplification for the ultrasensitive detection of AFB1 in water bodies.