Urease‐Mediated Reversible Calcium Carbonate Mineralization for Single Living Yeast Cell Nanoencapsulation

. Because urease covalently bonds to the yeast cell surface, reversible mineralization is achieved through EDTA-induced decomposition and urease-induced remineralization. In addition, the encapsulated urease endowed the engineered cell with catalytic sites for urea utilization, demonstrating the multifunctionality of the shell. This strategy realizes enzyme-catalyzed reversible mineralization of single living cells and demonstrates its potential in the construction of multifunctional shells, providing a nanoscale tool for structural modification to obtain single-cell factories for application in bioengineering and bio-manufacturing.