肝细胞癌
细胞培养
倍增时间
免疫分型
肝癌
生物
病理
体内
核型
癌症研究
流式细胞术
分子生物学
医学
遗传学
染色体
基因
作者
Hao Xu,Xin Miao,Chao Chai,Huan Tang,Jun Hu,Zhenjie Zhao,Wei Luo,Kun Zhu,Wence Zhou
出处
期刊:Human Cell
[Springer Nature]
日期:2022-09-24
卷期号:36 (1): 434-445
被引量:2
标识
DOI:10.1007/s13577-022-00797-z
摘要
Hepatocellular carcinoma (HCC) is a highly aggressive and heterogeneous disease. Cell lines are commonly employed as in vitro models for cell type studies. However, the success rate of HCC primary culture establishment is low. In this study, we successfully established a liver cancer cell line, Hep-X1. Primary culture and passage of surgically removed tissues were used to establish hepatoma cell lines. Morphological examination, short tandem repeat (STR) analysis, immunohistochemical staining, doubling time, karyotype analysis, plate tumor formation experiments, organoid culture, and in vivo tumor formation investigations in animals were used to identify the cell lines. A novel liver cancer cell line, Hep-X1, was established based on morphology, immunophenotype, cytogenetics, and STR analysis. The novel cell line can be a valuable model for studying primary liver cancer.
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