Detection and genotyping of Campylobacter jejuni and Campylobacter coli in food samples by fla-DGGE.

The aim of this study was to apply a denaturing gradient gel electrophoresis assay of the flagellin gene(fla-DGGE) for detection and genotyping of Campylobacter jejuni and Campylobacter coli in food samples without prior cμLtivation.RBB(rpeated bead beating),CTAB and acetone-chloroform method were used to extracted DNA of all the samples,and then fla-DGGE was applied.The resμLts of the three methods were the same.10 samples,8 were with Campylobacter jejuni or Campylobacter coli,which 3 samples contaminated by two or more of the Campylobacter jejuni or Campylobacter coli,or their different types.After cloning and sequencing resμLts showed that 7 samples were contaminated with the same Campylobacter jejuni,including 3 samples also have the same type of Campylobacter coli,a serious pollution samples were detected to contain one Campylobacter coli and three types of Campylobacter jejuni.Fla-DGGE method is rapid,accurate,and sensitive and could be used in food in Campylobacter jejuni and Campylobacter coli in the rapid detection and typing.