Saccharomyces cerevisiae and S.fragrns immobilized with PVA as carrier were used to produce seaweed health wine by double bottom fermentation.Through the study of batch fermentation and continuous fermentation of free cells and immobilized cells,the corresponding fermentation kinetic equation was set up.The experimental results suggested that the optimal fermentation conditions were as follows: the optimal ratio of Saccharomyces cerevisiae and Sacchromyces fagrans for inoculation as 4∶1;fermentation temperature at 20 ℃;packing ratio of the gel particles for batch fermentation and continuous fermentation were 0.25 and 0.5 respectively;7 d fermentation time for free cells and the dilution rate for continuous fermentation as 0.12/h with fermentation time about 0.5 d.The mechanical strength of the immobilized cell particles remained in good conditions after 160 d continuous fermentation.(Tran.by YUE Yang)