Chemical constituents of Phyllanthus amaus

Objective To study the chemical constituents of Phyllanthus amarus.Methods Chromatography on silica gel column,Sephadex LH-20 columm,recrystallization,and preparative HPLC technique were used to isolate and purify the compounds.Spectroscopic methods including UV,IR,EI-MS,1H-NMR,13C-NMR,HMQC,and HMBC were used to elucidate the structures of compounds.Results Nine compounds were obtained and identified as palmitic acid(1),4-hydrobenzal dehyde(2),oleanolic acid(3),stigmast-5-en-3-ol,oleate(4),4-hydroxy-3-methoxy-benzoic acid(5),daucosterol(6),apigenin(7),luteolin(8),and phyllanoside(9).Conclusion Compound 9 is a new compound,named phyllanoside.Compounds 4—8 are isolated from the plant for the first time.