Through the different withering and drying method on Ginkgo Biloba leaves to study the bio-active constituents of Ginkgo Biloba leaves.Results show that when Ginkgo Biloba leaves is withering in 30 ℃ sunshine for 5 h,and then 70 ℃ drying,the yield rate of gingko flavonoids is 1.72%,the yield rate of ginkgolic acids is only 0.58%,ginkgolic acid content decreased 69%.