Application of molecularly imprinted solid-phase extraction for separating and assaying amphetamines in blood samples

Objecive A method for extraction of amphetamines in blood samples using molecularly imprinted solid phase extraction and gas chromatography-mass spectrometry(GC/MS).Methods Human blood samples were spiked with amphetamine,methamphetamine,MDA and MDMA,and diluted with 4∶1(v/v)with 10mmol/L ammonium acetate buffer(pH8.0);activating the MIP column with 1mL methanol and 1mL 10mmol/L ammonium acetate(pH8.0);Washing the impurities using 2 1mL water,1mL 60/40 MeCN/water and 1mL 1% HAc in MeCN;Elute the amphetamine drugs with 2 1mL 1% formic acid in methanol,then evaporate under nitrogen to dryness and reconstitute with 100μL methanol prior to GC/MS analysis.Results The recoveries obtained with this method for amphetamine drugs are more than 90%.The linear range was 20～5000ng/mL with correlation coefficients between 0.995 7 and 0.998 9.The limits of detection were 20ng/mL for amphetamine(AM),16ng/mL methamphetamine(MA),30ng/mL methylenedioxyamphetamine(MDA)and methylened ioxy methamp hetamine(MDMA),The limits of quantitative were 10ng/mL for AM,8ng/mL for methamphetamine(MAM),15ng/mL for MDA and MDMA.Conclusion Recoveries are higher,impurities were less.The method could be applied for simultaneous determination of trace amphetamines in biological specimens.