Determination of Amygdalin in Apricot-kernel

Amygdalin in Apricot-kernel was determinated by a RP-HPLC method.This work was performed on a Shimadzu LC-4A HPLC with SPD-2AS UV Detector and a C 18 column (5μm,15cm×4.6mm ID).The mobile phase was methanol.water(3∶7,V/V).the standard curve was linear in the rage of 4.08～32.6μg for amygdalin.The recovery of amygdalin was 970%(n=5) and RSD was 1.63%.