The content of piperine in different pepper was studied.HPLC and UV methods were used to determine the content of piperine in different Pepper(black pepper,pepper root,sell pepper and white pepper peeled by different steep).The results showed that the content of peperine in the fruit is more than that in the root,while that in the white pepper is more than that in the dark pepper,and that in the pepper from Xi'an is more than that from Hainan.The content of the peperine is the most when brine was used as steep,and the lest was got when vinegar was employed.The content of piperine is different in different pepper,which can afford useful references to the quality control of pepper.