Flammulina velutipes is a kind of edible mushroom widely cultivated in China,with high value of medicinal and food therapy. Ergothioneine,which can be used as food antioxidant,is a natural antioxidant substance rich in Flammulina velutipes extracts. However,ergothioneine has been hardly investigated in China. In this article,the characteristics,determination methods and application prospects in food industry of ergothioneine in the extracts from Flammulina velutipes were introduced,and its significance for lengthening the shelf life of fresh meat and improvement of meat quality was proposed.