Antimicrobial activity of PVC-pyrazolone-silver nanocomposites

Poly(vinyl chloride)-pyrazolone; PVC-pz as a new modified form of PVC with high antibacterial and antifungal activities towards some Gram +ve bacteria S. Aureus, B. Subtilis and S. Faecalis) and Gram –ve bacteria (E. Coli, P. Aeruginosa and N. Gonorrhoeae) beside Candida albicans in addition to Aspragillus flavus as fungi has been synthesized in absence and in presence of silver-nanoparticles, AgNPs, in 3 and 5% by weight with respect to PVC. This was developed by a chemical reaction of PVC 1, and pyrazolone 2 in tetrahydrofuran, THF as solvent. PVC-pz derivative has been characterized by FTIR, 1HNMR spectroscopic analyses, in addition to scanning and transmission electronic microscopy. Photostability of PVC-pz was evaluated by following the discoloration for UV- irradiated samples colorimetrically in accordance with degradation time. IR and 1H-NMR analyses confirm that PVC-pz, 4 was formed indicating a substitution reaction of chlorine atoms of PVC chains to the hydrogen atom of active methylene and that attached to pyrazolone nitrogen at the same time. Antimicrobial activities of PVC-pz increased in the presence of AgNPs and with increasing its percentage.