Protel99SE is an all in one electronic design automation (EDA) software with a set of design tools,which can not only achieve the schematic circuit and PCB board production,but also provide users with powerful,easy-to-use circuit simulation. It can be used for the simulation,analysis and verification of the schematic circuit,reflecting the performance of the designed circuit immediately. In this paper,the main characteristics of Protel99SE and the general steps of circuit simulation are presented,the specific methods of analog circuit simulation analysis are discussed with a simple rectifier circuit regulator as an example,matters needing attention when drawing the schematic circuit are emphasized.