Chemical constituents of Caulophyllum robustum Maxim

AIM To study the chemical constituents of Caulophyllum robustum Maxim.METHODS Caulophyllum robustum Maxim was extracted by alcohol at reduced pressure,extract obtained was mixed with water and then extracted with petroether,ethylacetate,chloroform,n-butanol.Compounds 1-4 were isolated with silica gel and Sephadex LH-20 gel from the n-butanol extract part.Compounds 5-7 were isolated with silica gel from the ethyl acetate extract part.Their structures were elucidated by spectroscopic and chemical methods.RESULTS Seven compounds were identified as echinocysticacid-3-O-α-L-arabinopyranoside(1),hederagenini-3-O-β-D-glucopyranosyl-(12)-α-L-arabino-pyranoside(2),Hederag enin-3-O-α-L-arabinopyranoside(3),Caulophyllogenin-3-O-α-L-arabino-pyranoside(4),β-sitgmasterol(5),lupeol(6),cholesterol(7).CONCLUSION Compound 1 is isolated from Caulophyllum for the first time and compounds 5,6 and 7 are isolated from this plant for the first time.