Infection Process of Curvularia lunata on Corn Leaf Observed in Incompatible Interaction

Infection process of Curvularia lunata on corn leaf were observed by means of tissue transparent method.The results showed that the conidia of Curvularia lunata germinated at 2 h after inoculation.The germination percentages of conidia examined at 4 h and 6 h after inoculation were 80% and 99%,respectively.Invasive hypha penetrated epidermal cell through intercellular space and stoma between 12 h and 24 h.Invasive hypha became thin when it penetrated cell wall.Structure of stoma was destroyed and guard cells were degraded when invasion hypha pierced the stomas.Conidiophore and conidia stretched form intercellular space and stomas on 3days after inoculation.Corn leaves appeared typical symptom on 3 days after inoculation.