The leaf reflectance spectra (R), lg(1/R), standard first derivative reflectance spectra (FD) and absorption band depths following the continuum removal and normalization (BN) of Changyou 1 and Wujing 15 were measured and calculated. The relationship between different spectral performances and rice leaf chlorophyll contents was analyzed. Then the statistic equations between leaf chlorophyll contents and different spectral performances were established, and a comparison was made between them. The best estimator selection was also discussed. The results show that the correlation obtained by the equation established from FD is the best, and the lg (1/R) spectra can improve the retrieval of chlorophyll contents with remote sensing. The multiple correlation coefficients (R2) between estimated and observed rice leaf chlorophyll contents of the two varieties were 0.641 and 0.818 respectively.