Objective To investigate the surgical treatment and therapeutic effect of laparoscopic peritoneal vaginoplasty on congenital absence of vagina.Methods The clinical data of 44 patients with congenital absence of vagina receiving laparoscopic peritoneal vaginoplasty were retrospectively analyzed.The operation time,blood loss volume and postoperative function of vagina were observed.Results Forty-three patients(97.7%)received successful operations.The operation lasted(63.40±13.98)minutes,and the blood loss volume was(74.25±17.75)mL.The time to passage of gas by anus was(2.40±0.89) days.All patients were followed up for(7.65±2.23)months.The length of vagina was(8.80±1.30)cm.Artificial vagina mucosa was smooth,ruddy and flexible,which was consistent with anatomical structures and could meet the physiological demand.Conclusion Laparoscopic peritoneal vaginoplasty is a safe,minimally invasive and reliable method for congenital absence of vagina.