Renaturation of the Inclusion Bodies Within Recombinant Protein

Overexpression of recombinant proteins in the Escherichia coli cytoplasm often results in the formation of insoluble inclusion bodies.Active protein can be recovered by solubilization of inclusion bodies followed by renaturation of the solubilized protein.The process of renaturation is established in the understanding to the mechanism of protein folding in vitro.According to the progression of protein folding research,three questions are reviewed:1) the protein folding in vivo;2)the mechanism of protein folding in vitro;3) the renaturation strategy.