OBJECTIVE: To investigate the optimum ethanol extraction process conditions for salidroside and p-tyrosol from Rhodiola crenulata. METHODS: The extraction rate of ethanol extract and content of salidroside and p-tyrosol were chosen as the assessment index by using the orthogonal design. The ethanol content (%), solvent quantum (fold) and extracting time (hour) were reviewed. RESULTS: The optimum ethanol extraction process conditions were as follows: adding 6 fold of 80% ethanol, extracting for 2h and 2 times. CONCLUSION: The optimum extraction process can give a reference to the research of new medicine and production of industry.