Isolation and characterization of bacteria associated with a syndrome disease of sea urchinStrongylocentrotus intermediusin North China

Aquaculture ResearchVolume 44, Issue 5 p. 691-700 Original Article Isolation and characterization of bacteria associated with a syndrome disease of sea urchin Strongylocentrotus intermedius in North China Yinan Wang, Yinan Wang Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorNisha Feng, Nisha Feng Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorQiang Li, Qiang Li Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorJun Ding, Jun Ding Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorYaoyao Zhan, Yaoyao Zhan Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorYaqing Chang, Corresponding Author Yaqing Chang Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaCorrespondence: Yaqing Chang, Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture; Dalian Ocean University, Dalian, China. E-mail:yaqingchang@hotmail.comSearch for more papers by this author Yinan Wang, Yinan Wang Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorNisha Feng, Nisha Feng Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorQiang Li, Qiang Li Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorJun Ding, Jun Ding Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorYaoyao Zhan, Yaoyao Zhan Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaSearch for more papers by this authorYaqing Chang, Corresponding Author Yaqing Chang Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, ChinaCorrespondence: Yaqing Chang, Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture; Dalian Ocean University, Dalian, China. E-mail:yaqingchang@hotmail.comSearch for more papers by this author First published: 20 January 2012 https://doi.org/10.1111/j.1365-2109.2011.03073.xCitations: 35Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Sea urchin, Strongylocentrotus intermedius, transplanted from Japan in 1989, has been widely cultured along the coasts of Liaoning and Shandong Provinces and has become the dominant and most economically important maricultured species in North China. However, a lesion syndrome symptom of S. intermedius broke out frequently these years, showing lethargy in activities, blackish peristomial membrane and body well lesions, and brought about high mortality eventually. Six representative prominent bacterial strains were isolated from diseased sea urchin from September 2009 to January 2010. By means of API 20NE and 16S rRNA gene sequences analysis, isolates were identified as Shewanella aquimarina, Pseudoalteromonas tetraodonis, Vibrio shilonii, V. harveyi, V. fortis and V. splendidus. Bacterial challenge tests showed that their representative isolates were virulent to S. intermedius with LD50 values ranging from 9.2 × 104 to 3.4 × 106 CFU/g body weight, among which S. aquimarina , V. fortis and P. tetraodonis were highly virulent, and the other three isolates showed moderate virulence. The results indicated that a variety of bacteria including Shewanella, Pseudoalteromonas and Vibrio were involved in the mortality of S. intermedius, and the six isolates were opportunistic pathogens of sea urchins. All isolates reported herein were sensitive to ampicillin, enrofloxacin, ofloxacin, doxycycline and florfenicol. Citing Literature Volume44, Issue5April 2013Pages 691-700 RelatedInformation