Genetic analysis of artificial pigmentation mutants in Porphyra yezoensis Ueda (Bangiales, Rhodophyta)

SUMMARY Porphyra yezoensis Ueda artificial pigmentation mutants, yel (green), fre (red-orange) and bop (pink), obtained by treatment with /V-methyl-/V′-nitro-N-nitrosoguanidine, were genetically analysed. The mutations associated with color phenotypes are recessive because all of the heterozygous conchocelis resembled the wild type color when they were crossed with the wild type (wt). In the reciprocal crosses of yel × wt, both parental colors and eight types of blades appeared in the F1 gametophytic blades from the heterozygous conchocelis. Both colors segregated in the sectored F1 blades in a 1:1 ratio, indicating that the color pheno-type of yel resulted from a single mutation in the nuclear gene. In the reciprocal crosses of fre × wt, however, four colors and more than 40 types of blades appeared in the F1 blades from the heterozygous conchocelis, indicating that the color phenotype of fre resulted from two mutations in different genes. In the reciprocal crosses of bop×wt, three colors and 12 types of blades were observed in the F1 blades from the heterozygous conchocelis. Both parental colors appeared far more frequently than the third new color. These results indicated that the color phenotype of bop resulted from two closely linked mutations in different genes, and the epistasis occurred in the F1 blades. The mutants, yel, fre and bop, differ from the spontaneous green (C-O), the red (H-25) and the violet (V-O) mutants of P. yezoensis, respectively.