丙酸盐
发酵
拟杆菌
普雷沃菌属
多糖
消化(炼金术)
丁酸盐
淀粉
食品科学
微生物代谢
脆弱类杆菌
阿魏酸
拟杆菌科
细菌
生物化学
生物
肠道菌群
新陈代谢
微生物学
化学
抗生素
遗传学
色谱法
作者
Mark Hopkins,Hans N. Englyst,Sandra Macfarlane,Elizabeth Furrie,G.T. Macfarlane,Andrew J. McBain
标识
DOI:10.1128/aem.69.11.6354-6360.2003
摘要
ABSTRACT In humans, nonstarch polysaccharides (NSP), such as arabinoxylans (AX), are not digested in the upper gut and provide fermentable carbon sources for bacteria growing in the large bowel. Despite the ubiquity of AX in nature, the microbiologic and physiologic consequences of AX digestion in the gut are poorly understood. In this study, we investigated the breakdown of ferulic acid-cross-linked AX (AXF) and non-cross-linked AX in children's intestinal microbiotas, using starch as a readily fermentable polysaccharide for comparative purposes. The experiments were performed using pH-controlled fermentation vessels under anaerobic conditions. The results demonstrated that there was variation in the metabolism of these polysaccharides by colonic microbiotas. AX was always degraded more slowly than starch, while ferulic acid cross-linking reduced the rate of AX fermentation, as shown by fermentation product measurements. Starch digestion was associated with significant acetate and butyrate production, whereas AX breakdown resulted in increased propionate formation. In general, the presence of fermentable carbohydrate significantly increased the total anaerobe counts and eubacterial rRNA concentrations ( P < 0.01), while non-cross-linked AX digestion was principally associated with increased viable counts of Bacteroides fragilis group organisms, which was supported by increases in Bacteroides - Porphyromonas - Prevotella group rRNA ( P < 0.01). Starch was considerably more bifidogenic than AX in these fermentations. In conclusion, in this study we found that the effects of AX and AXF on the microbial ecology and metabolism of intestinal microbiotas are similar in children and adults.
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