放大器
聚合酶链反应
聚合酶链反应优化
热启动PCR
反聚合酶链反应
分子生物学
底漆二聚体
PCR的应用
硅胶PCR
基因组DNA
DNA
片段(逻辑)
聚合酶
多重位移放大
生物
化学
数字聚合酶链反应
计算生物学
套式聚合酶链反应
遗传学
多重聚合酶链反应
DNA提取
基因
计算机科学
程序设计语言
作者
Eng Wee Chua,Simran Maggo,Martin A. Kennedy
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 65-74
被引量:4
标识
DOI:10.1007/978-1-4939-7060-5_3
摘要
Polymerase chain reaction (PCR) is an oft-used preparatory technique in amplifying specific DNA regions for downstream analysis. The size of an amplicon was initially limited by errors in nucleotide polymerization and template deterioration during thermal cycling. A variant of PCR, designated long-range PCR, was devised to counter these drawbacks and enable the amplification of large fragments exceeding a few kb. In this chapter we describe a protocol for long-range PCR, which we have adopted to obtain products of 6.6, 7.2, 13, and 20 kb from human genomic DNA samples.
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