The cactus moth, Cactoblastis cactorum Berg, is an invasive species that threatens economically and ecologically important native cacti in Mexico and the U.S. Southwest. The insect presently occurs along the coastal U.S. from Charleston, SC, to Dauphin Island, south of Mobile, AL, and in the interior of Florida. Current control and eradication tactics include manual destruction of infested cactus and the Sterile Insect Technique (SIT), which involves the release of irradiated cactus moths to mate with wild moths and produce sterile offspring. The ability to rear the cactus moths in the laboratory has been crucial in research and development of survey and control techniques. Procedures for rearing the cactus moth in the laboratory on cactus cladodes and artificial diet are described and provide a foundation for the further development of mass-rearing protocols.