Preparation of Nanocomposite Silver-Chitosan-Alginate Film as Antibacterial Material

Abstract Preparation of silver-chitosan-alginate (Ag/Kit/Alg) nanocomposite film as an antibacterial material has been done. Nanocomposite film of Ag/Chit/Alg was carried out through casting method of colloidal nanocomposite Ag/Chit/Alg). Colloidal is made through a chemical reduction method of AgNO 3 precursor salts assisted by sunlight with chitosan as a stabilizer agent and reducing as well and NaOH as an accelerator. Furthermore, alginate solution is added to form Ag/Kit/Alg nanocomposite colloidal. The formation of silver nanoparticles was indicated by the appearance of localize surface plasmon resonsnce (LSPR) phenomenon which was characterized using a UV-Vis spectrophotometer. The shape and size of silver nanoparticles were characterized using TEM. Characterization of Ag/Kit/Alg nanocompsite films includes swelling, mechanical properties, solubility and water vapor permebility (WVP). Testing of antibacterial activity was carried out on film Ag/Chit/Alg nanocomposit using diffusion method. The results showed that the formation of silver nanoparticles was characterized by the appearance of LSPR phenomenon at the peak of 403.50 - 412.50 nm. Silver nanoparticles are spherical shape with a size dominated at 5-9 nm. The physical and mechanical properties of films are influenced by the concentration of silver nanoparticles. The greater concentration of precursor salt AgNO 3 increase swelling and solubility of Ag/Chit/Alg nanocomposte film. The tensile strength of the film tends to decrease due to the presence of Ag nanoparticles. The nanocomposite film of Ag/Chit/Alg has a rough structure with a higher Water Vapor Permeability value compared to Ag/Chit films. The nanocomposit films of Ag/Kit/Alg have high antibacterial activity against Escherechia coli ( E. coli) ATCC 25922 and Staphylococcus aureus (S. Aureus) ATCC 25923.