脐带血
医学
重复性
标准化
男科
免疫学
化学
计算机科学
色谱法
操作系统
作者
Diane Fournier,Antoine Lewin,Carl Simard,Patrick Trépanier,Sonia Néron,Lara Ballerini,Margarita Codinach,Heidi Elmoazzen,Mike Halpenny,Gesine Kögler,Stefanie Liedtke,Isabelle Louis,Carmen Azqueta Molluna,Nicolas Pineault,Arun Prasath,Sergio Querol,Riccardo Saccardi,D. Robert Sutherland,Cynthia Thérien,Serena Urbani
出处
期刊:Cytotherapy
[Elsevier]
日期:2020-01-01
卷期号:22 (1): 44-51
被引量:10
标识
DOI:10.1016/j.jcyt.2019.10.009
摘要
Background aims In 2016, specifications for both pre-cryopreserved and post-thawed cord blood were defined in the sixth edition of NetCord Foundation for the Accreditation of Cellular Therapy (FACT) Standards for Cord Blood Banks. However, for several experts, harmonization regarding flow cytometry analysis performed on post-thawed samples is still a concern. A multicenter study led by Héma-Québec aimed to provide scientific data to support the cord blood accreditation bodies such as NetCord FACT in the revision of standards. Methods Twelve cord blood units were processed for plasma and red cell reduction following standard operating procedures. Cord blood unit aliquots were shipped to eight participating centers under cryogenic conditions for analysis before and after standardization of protocol. Repeatability of stem cell count, measured pre- and post-intervention with the centers, was estimated using multilevel linear regression models with a heterogeneous compound symmetry correlation structure among repeated measures. Results Excellent inter-center repeatability was reported by each participant regarding the viable CD34+ cells concentration, and a successful improvement effect of protocol standardization was also observed. However, we observed that better control over the critical parameters of the protocol did not have a significant effect on improving homogeneity in the enumeration of CD45+ cells. Conclusions The current practice in cord blood selection should now also consider relying on post-thaw CD34+ concentration, providing that all cord blood banks or outsourcing laboratories in charge of the analysis of post-thaw CB samples take into account the consensual recommendations provided in this work and adhere to a good-quality management system.
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