Clone library analysis of Lactic acid bacteria composition in ICR mice

Objective To study the composition of Lactic acid bacteria in the intestine of healthy ICR mice.Methods Fresh fecal samples from 10 healthy male ICR mice were collected and the total genomic DNA was extracted from each sample.Lactic acid bacteria(LAB) group specific primer(Lac1) and universal primer for bacteria(1391r) were used to amplify 16S rRNA gene and construct LAB group specific clone library to analyze the species composition of LAB.Results The results of clone library showed that Lactobacillus reuteri and Lactobacillus johnsonii were the most predominant species in gut of mice.Lactobacillus murinus,Lactobacillus vaginalis,Lactobacillus intestinalis and a potentially new species were also detected.Conclusion The composition and structure of Lactobacillus in healthy ICR mice have high diversity and the species of Lactobacillus reuteri may have high microdiversity at strain level.