Lipoxin A4 Inhibits Lipopolysaccharide-induced Differentiation of RAW264.7 Murine Macrophages into Dendritic-like Cells and Its Maturation

Aim To investigate the effects of lipoxin A4(LXA4) on lipopolysaccharide(LPS)-induced differentiation and mature of RAW264.7 macrophages into dendritic like cells(DC).Methods RAW264.7 murine macrophages were respectively treated with LPS(0 μg/L and 100 μg/L),LPS(100 μg/L) together with LXA4(100 nmol/L and 500 nmol/L).Cells surface markers(CD80,CD86 and MHC-II) were analyzed by FACS,and cell morphology was observed by laser scanning confocal microscope and phase contrast microscope.Results LPS could significantly promote the differentiation of RAW264.7 murine macrophages into DC and the expression of surface molecules(CD80,CD86,MHC-II)(P0.01),and LXA4 could inhibit the differentiation of LPS-stimulated RAW264.7 murine macrophages and down-regulate the expression of CD86 and MHC-II,but had no significant effect on the expression of CD80.Conclusion LXA4 could negatively regulate the differentiation of RAW264.7 into DC and inhibit the maturation of DC,which may inhibit the pathogenesis and development of atherosclerosis by negatively regulating immuno-inflammatory response.