Toxic effects of 1,4-dichlorobenzene(1,4-DCB) on Chlorella pyrenoidosa were studied through the changes of growth,the protein contents,and the chlorophylla contents of algae.And the toxic mechanisms of Chlorella pyrenoidosa were analyzed by the activities of SOD enzymes and the MDA contents of algae.The results showed that the growth of algae was inhibited by the 1,4-DCB in treated time-response and dose-response manners.The protein contents,the chlorophylla contents,especially the contents of the chlorophylla a,were decreased in Chlorella pyrenoidosa cells treated by 1,4-DCB for 4 d.The activities of SOD were decreased and the contents of MDA increased in dose-response manners.The results suggested that primary mechanism of 1,4-DCB on Chlorella pyrenoidosa cells may be related to the oxidation damage of lipid and other biological large molecules caused by 1,4-DCB.The pollution of 1,4-DCB decreased the values of Chlorella pyrenoidosa as the food of organisms.