Optimization on culture conditions of Rhizobia in different areas

Environment of soil is the primary factor affected nodulation of Rhizobia,and its type,pH as well as other physical and chemical properties can influence the effect of Rhizobia inoculation.Therefore,it is necessary for Rhizobia from different regions to optimize culture conditions in order to ensure that the inoculation of Rhizobia can produce a large number of effective nodules,reach high production purposes.Rhizobia from Hailun,Suiling,Beian,Hulan and Suihua were investigated for obtaining the optimal culture conditions by single factor.The results showed that the optimal culture conditions were as below:culture time was,48,48,48,96 and 48 h,respectively;initial pH was 6.0,7.5,6.5,8.0,6.5,respectively;rotate speed was 180,180,160,180,180 r·min-1,respectively;culture temperature of all the Rhizobia was 28 ℃.