Clone and Expression Characterization of CsPEAMT Gene in Cleistogenes songorica

Based on the highly homologous EST sequence of phosphoethanolamine N-methyltransferase(PEAMT)gene obtained from drought-induced cDNA library of Cleistogenes songorica,a full-length cDNA of PEAMTgene was isolated fromC.songorica.Full length of CsPEAMT was 2104 bp,containing a 1506 bp ORF and encoding 501 amino acids.Comparative analyses revealed that the amino acid sequence encoded by CsPEAMT gene was highly homologous to PEAMTs from other plant species,and CsPEAMT had the highest similarity with that of Sorghum bicolor and Zea mays(93%).The expression patterns of CsPEAMTduring drought stress were investigated by real-time quantitative RT-PCR analysis.The results showed that drought stress induced a large number of the gene expression in root and leaf.And the CsPEAMTexpression level in leaf and root are 43.35 times and 13.25 times higher than that of control,respectively,then the expression level began to decrease afer rewatering.This study indicated that CsPEAMT may be a drought-related gene.