Analysis of Mixed Emulsion of Avermetin B1 and Phoximby HPLC

High efficiency chromatography was adopted for the separation and determination of avermectin B1 and phoxim in mixed emulsion on the Lichrospher-C18 column with methanol+water(85+15)as mobile phase and UV detector.Under the selected conditions,the retention times of avermectin B1 and phoxim were 28.5min and8.1 min respectively.RSD's(n=6) for avermectin B1 and phoxim were found to be 0.74% and 1.52%.And the average recoveries were 100.2% and 100.3%.The linear correlation coefficients were 0.9985 and 0.9989respectively.