Optimization of regeneration system and resistance screening of Rhodiola crenulata

Objective To optimize the regeneration system of Rhodiola crenulata,to establish the optimal conditions for resistance screening,and to lay the foundation for the establishment of the efficient genetic transformation system of R.crenulata.Methods The leaves of Rhodiola crenulata were used as explants.The influences of different ratios of 6-BA,NAA,and IAA in the medium on callus induction and growth conditions were observed,and the implant resistance of Kanamycin(Kan) and hygromycin(Hyg) was screened by gradient method.Results MS + 3.0 mg/L 6-BA + 1.0 mg/L NAA + 700 mg/L L-Pro was the optimal medium for the differentiation of leaf adventitious bud and the differentiation ratio of adventitious bud reached 92%;MS + 700 mg/L L-Pro was the medium for adventitious roots;The best selection of genetic transformation system for R.crenulata was 200 mg/L Kan and 10 mg/L Hyg;Adding 10 mg/L Vc could effectively inhibit the phenolic substances secretion.Conclusion The regeneration system of R.crenulata,is optimized,and the pressure of Kan and Hyg for genetic transformation system of R.crenulata is screened.