Preparation and Identification of Monoclonal Antibody Against Candida albicans and the Influence Caused by McAb to Germ TubesInduction

Objective To develop and identify monoclonal antibodies against germ tubes of Candida albicans(C.albicans).Methods Blastospore of C.albicans ATCC-90028 were used as immunogens to prepare monoclonal antibodies(McABs),which were prepared by the lymphocyte hybridom technique,and were identificated.The result of germ tubes induction,which were induced by NCS,and with the paiticipation of McAb against C.albicans germ tubes,were observed.Results One celling of hybirdomase-creating constantly McAbs against germ tubes of C.albicans,which named McAb03.2C1-C2,was developed.The titer of McAb reached to 1∶25 600;The McAb belonged to IgG1 Lambda isotypes.The result that McAb03.2C1-C2 could specific react to the protein(156kD) antigen of germ tubes of Candida albicans was manifested.IIF diplaid that McAb03.2C1-C2 of mice hydroperitonia could specificly reacted to germ tubes of Candida albicans,but failed to react to phas of spore.Inductive radio of C.albicans germ tubes,which inducted by medium with the addition of McAb against C.albicans germ tube,was lower than inductive radio of C.albicans germ tube inducted by serum.Conclusion It is suggested that McAb03.2C1-C2 could be highly specific McAb against pathtype of Candida albicans-germ tubes,and it might be used in early and quick diagnoses of Systemic of C.albicans with a new prospect.McAb McAb03.2C1-C2,which could inhibited distinctly the diversion of C.albicans,can be a protective factor during the procress of resisting infection of systemic C.albicans.