Research on Adding Precursor Decanoic Acid for Fermentation of Daptomycin

In process of daptomycin fermentation,the research on initial feeding time,feeding amount,couplant and variable speed feeding of precursor decanoic acid via feeding decanoic acid by feeding rocking bed was conducted.It was concluded that the optimum initial feeding time of decanoic acid is h36 after fermentation,the optimum feeding amount of decanoic acid:methyloleate(1∶1) is 70 μL/12 h,the best couplant of decanoic acid is alcohol and the ratio was 2.5∶1.Varying feeding speed may raise the production of daptomycin,but not a lot.