无刺蜂
氯化钴
蜜蜂科
钴
幼虫
生物
蛹
氯化物
植物
化学
动物
分子生物学
膜翅目
有机化学
无机化学
作者
Carlos Ueira-Vieira,Rosendo Tavares,Silvia Morelli,Boscolli Barbosa Pereira,R.P. Silva,Alessandra Ribeiro Torres-Mariano,Warwick Estevam Kerr,Ana Maria Bonetti
出处
期刊:Genetics and Molecular Research
[Genetics and Molecular Research]
日期:2013-01-01
卷期号:12 (2): 2032-2037
被引量:1
标识
DOI:10.4238/2013.february.6.1
摘要
In order to optimize preparations of bee metaphases, we tested cobalt chloride, which has been used as a metaphase inducer in other organisms, such as hamsters and fish.Four microliters of 65 mM cobalt chloride aqueous solution was topically applied to larval and pupal stages of the stingless bee Melipona scutellaris.The cerebral ganglion was removed after treatment and prepared for cytogenetic analysis.Identically manipulated untreated individuals were used as controls.The number of metaphases was increased 3-fold in treated individuals compared to controls.The micronucleus test showed no mutagenic effects of cobalt chloride on M. scutellaris cells.We concluded that cobalt chloride is a metaphase-inducing agent in M.
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