Investigation on in vitro anti-influenza virus activity of Qingre mixture

OBJECTIVE To investigate the anti-influenza A3 effect of Qing Re He Ji(QRHJ)mixture.METHODS The techniques of chick embryo culture were used for evaluating antiviral activity of serum from the Wistar rats dosed with QRHJ by hemagglutination assay with the influenza virus hemagglutination titer as index.RESULTS The first,second,third and fouth hour's serum of the Wistar rats after oral administration of QRHJ by 24 g·kg-1 dose had antiviral effect.The inhibition rates(%)of hemagglutination titer were 86.37,82.32,80.72 and 72.74,respectively.The first,second and third hour's serum of the Wiistar rats after oral administration of QRHJ by 12 g·kg-1dose had antiviral effect.The inhibition rates(%)of hemagglutination titer were 71.53,67.58 and 66.14.QRHJ was found to be an inhibitor of influenza A3 virus in a concentration-dependent manner.CONCLUSION QRHJ has significant effect on influenza virus type A3 in the chick embryo.