Two strains that can transform linoleic acid to conjugated linoleic acid were screened from soil.The morphology,growth curves and the optimum conditions for CLA production in MRS medium were studied in this paper.Though the two strains were different significantly in morphology,the optimum conditions for CLA production of them were similar: concentration of linoleic acid was 0.51%(v/v),and incubation time was 36~48 h.