Comparison of Four Methods for Apoptosis Assay by Flow Cytometry

[Objective]The research aimed compare the advantages and disadvantages of four methods for apoptosis assay. [Method]Flow cytometry was used to examine cell apoptosis which labeled with PI,Annexin V-FITC /PI,Rhodamine 123 and Fluo-3 respectively. [Result]Each menthod had its merits and drawbacks,and multi-parameter analysis should be considered in the inspection process. [Conclusion]It could provide experimental support for sequential studies.