生物
白细胞介素12
Janus激酶3
细胞生物学
细胞
白细胞介素21
CD49b
神经细胞粘附分子
淋巴因子激活杀伤细胞
免疫学
细胞粘附
免疫系统
体外
细胞毒性T细胞
T细胞
遗传学
作者
Katy Wendt,Esther Wilk,Sabine Buyny,Jan Buer,Reinhold Schmidt,Roland Jacobs
摘要
Abstract Recent findings underline the role of NK cell subsets in regulating adaptive immunity. To define characteristics of NK cell subpopulations, purified CD56dim and CD56bright NK cells were analyzed by using gene chip arrays covering more than 39,000 transcripts. Gene profiling revealed resting NK cells to differ in respect to 473 transcripts with 176 exclusively expressed in CD56dim and 130 solely in CD56bright NK cells. Results were compared with array analyses using mRNA obtained from activated CD56dim and CD56bright NK cells. In this approach, NK cell receptors, cytolytic molecules, adhesion structures, and chemokine ligands showed differential expression patterns in the two subpopulations. These data were validated using FACS, RT-qPCR, or cytokine bead array (CBA) techniques. Cytokines produced by CD56dim and CD56bright NK cells were determined using a protein array covering 79 different bioactive mediators. GDNF, IGFBP-1, EGF, and TIMP-2 were detected in both subsets. In contrast, IGFBP-3 and IGF-1 were mainly produced by CD56dim, while GM-CSF, TARC, and TGFβ3 were expressed by CD56bright NK cells. In summary, we report new characteristic features of CD56dim and CD56bright NK cells, further underscoring that they represent independent populations with functionally diverse capabilities. The information on NK cells generated in this study will help to define corresponding NK cell populations in other species that lack CD56 expression on NK cells, such as mice. This will subsequently lead to the establishment of suitable animal models for detailed analysis of NK cell populations in vivo.
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