A halide perovskite/lead sulfide heterostructure with enhanced photoelectrochemical performance for the sensing of alkaline phosphatase (ALP)

For the construction of halide perovskite-based photoelectrochemical (PEC) biosensors in aqueous solution, the balance between retaining the excellent photoelectric performances of the halide perovskite and the protective modification of the halide perovskite has always been a challenging problem. In this work, a simple and sensitive photoelectrochemical biosensor based on inorganic halide perovskite CsPbBr₃ as the photoactive material for the detection of alkaline phosphatase (ALP) was reported. The substrate sodium thiophosphate (Na₃SPO₃) can be catalyzed by ALP to produce hydrogen sulfide (H₂S), which can react with the lead site on the surface of the CsPbBr₃ film to form lead sulfide (PbS), resulting in a stable heterostructure and enhanced photocurrent intensity. The possible mechanism of enhanced photocurrent response of CsPbBr₃/PbS heterojunctions was studied in detail. This work paves a new way for applying halide perovskites in different biosensor designs.