计算生物学
转录组
拉曼光谱
表征(材料科学)
生物
纳米技术
材料科学
遗传学
基因
物理
基因表达
光学
作者
Manuel Sigle,Anne-Katrin Rohlfing,Martin Kenny,Sophia Scheuermann,Na Sun,Ulla Graeßner,Verena Haug,Jessica Sudmann,Christian Seitz,David Heinzmann,Katja Schenke‐Layland,Patricia B. Maguire,Axel Walch,Julia Marzi,Meinrad Gawaz
标识
DOI:10.1038/s41467-023-41417-0
摘要
Spatial transcriptomics of histological sections have revolutionized research in life sciences and enabled unprecedented insights into genetic processes involved in tissue reorganization. However, in contrast to genomic analysis, the actual biomolecular composition of the sample has fallen behind, leaving a gap of potentially highly valuable information. Raman microspectroscopy provides untargeted spatiomolecular information at high resolution, capable of filling this gap. In this study we demonstrate spatially resolved Raman "spectromics" to reveal homogeneity, heterogeneity and dynamics of cell matrix on molecular levels by repurposing state-of-the-art bioinformatic analysis tools commonly used for transcriptomic analyses. By exploring sections of murine myocardial infarction and cardiac hypertrophy, we identify myocardial subclusters when spatially approaching the pathology, and define the surrounding metabolic and cellular (immune-) landscape. Our innovative, label-free, non-invasive "spectromics" approach could therefore open perspectives for a profound characterization of histological samples, while additionally allowing the combination with consecutive downstream analyses of the very same specimen.
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