滋养层
干细胞
白血病抑制因子
细胞生物学
胎盘形成
表观遗传学
生物
胚泡
染色质
转录组
胎牛血清
男科
胚胎干细胞
化学
细胞培养
胚胎
遗传学
胎盘
怀孕
基因
胎儿
胚胎发生
基因表达
医学
作者
Yinjuan Wang,Hao Ming,Lei Yu,Jie Li,Linkai Zhu,Hai‐Xi Sun,Carlos A. Pinzón-Arteaga,Jun Wu,Zongliang Jiang
出处
期刊:Cell Reports
[Elsevier]
日期:2023-05-01
卷期号:42 (5): 112439-112439
被引量:5
标识
DOI:10.1016/j.celrep.2023.112439
摘要
Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.
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