血栓造影术
凝血病
医学
代谢组学
蛋白质组学
血栓弹性测定
人口
休克(循环)
凝结
纤维蛋白原
失血性休克
组学
生物信息学
内科学
生物
生物化学
基因
环境卫生
作者
Ian S LaCroix,Alexis Cralley,Ernest E. Moore,Francesca Cendali,Monika Dzieciątkowska,Patrick Hom,Sanchayita Mitra,Mitchell J. Cohen,Christopher C. Silliman,Angela Sauaia,Kirk C. Hansen,Angelo D’Alessandro
出处
期刊:Annals of Surgery
[Ovid Technologies (Wolters Kluwer)]
日期:2023-06-19
卷期号:278 (6): e1299-e1312
被引量:2
标识
DOI:10.1097/sla.0000000000005944
摘要
Objective: Advanced mass spectrometry methods were leveraged to analyze both proteomics and metabolomics signatures in plasma upon controlled tissue injury (TI) and hemorrhagic shock (HS)—isolated or combined—in a swine model, followed by correlation to viscoelastic measurements of coagulopathy via thrombelastography. Background: TI and HS cause distinct molecular changes in plasma in both animal models and trauma patients. However, the contribution to coagulopathy of trauma, the leading cause of preventable mortality in this patient population remains unclear. The recent development of a swine model for isolated or combined TI+HS facilitated the current study. Methods: Male swine (n=17) were randomized to either isolated or combined TI and HS. Coagulation status was analyzed by thrombelastography during the monitored time course. The plasma fractions of the blood draws (at baseline; end of shock; and at 30 minutes, 1, 2, and 4 hours after shock) were analyzed by mass spectrometry-based proteomics and metabolomics workflows. Results: HS—isolated or combined with TI—caused the most severe omic alterations during the monitored time course. While isolated TI delayed the activation of coagulation cascades. Correlation to thrombelastography parameters of clot strength (maximum amplitude) and breakdown (LY30) revealed signatures of coagulopathy which were supported by analysis of gene ontology-enriched biological pathways. Conclusion: The current study provides a comprehensive characterization of proteomic and metabolomic alterations to combined or isolated TI and HS in a swine model and identifies early and late omics correlates to viscoelastic measurements in this system.
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