脊髓
神经干细胞
移植
神经突
祖细胞
脊髓损伤
神经科学
再生(生物学)
星形胶质细胞
轴突
胶质瘢痕
医学
解剖
干细胞
生物
中枢神经系统
细胞生物学
外科
体外
生物化学
作者
Jin Chen,Yayu Wu,Haipeng Zhang,Xu Bai,Wenbin Liu,Chunnan Ji,Panpan Li,Zhenni Chen,Bing Chen,Jiayin Li,Xianming Wu,Pengju Jiang,Yali Hu,Zhifeng Xiao,Yannan Zhao,Jianwu Dai
摘要
Neural progenitor cell (NPC) transplantation is a promising approach for repairing spinal cord injury (SCI). However, cell survival, maturation and integration after transplantation are still major challenges. Here, we produced a novel centimeter-scale human spinal cord neural tissue (hscNT) construct with human spinal cord neural progenitor cells (hscNPCs) and human spinal cord astrocytes (hscAS) on a linearly ordered collagen scaffold (LOCS). The hscAS promoted hscNPC adhesion, survival and neurite outgrowth on the LOCS, to form a linearly ordered spinal cord-like structure consisting of mature neurons and glia cells. When transplanted into rats with SCI, the hscNT created a favorable microenvironment by inhibiting inflammation and glial scar formation, and promoted neural and vascular regeneration. Notably, the hscNT promoted neural circuit reconstruction and motor functional recovery. Engineered human spinal cord implants containing astrocytes and neurons assembled on axon guidance scaffolds may therefore have potential in the treatment of SCI.
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