正电子发射断层摄影术
生物发光成像
间充质干细胞
体内
干细胞
碘化钠转运体
报告基因
临床前影像学
材料科学
磁共振成像
分子成像
磁粉成像
生物医学工程
纳米技术
医学
核医学
化学
磁性纳米粒子
细胞培养
转染
病理
纳米颗粒
放射科
细胞生物学
基因表达
生物
荧光素酶
基因
共转运蛋白
生物技术
遗传学
生物化学
运输机
作者
Nourhan Shalaby,John J. Kelly,Olivia C. Sehl,Julia J. Gevaert,Matthew S. Fox,Qi Qi,Paula J. Foster,Jonathan D. Thiessen,Justin Hicks,Timothy J. Scholl,John A. Ronald
出处
期刊:Nanoscale
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:15 (7): 3408-3418
被引量:2
摘要
Stem cell-based therapies have demonstrated significant potential in clinical applications for many debilitating diseases. The ability to non-invasively and dynamically track the location and viability of stem cells post administration could provide important information on individual patient response and/or side effects. Multi-modal cell tracking provides complementary information that can offset the limitations of a single imaging modality to yield a more comprehensive picture of cell fate. In this study, mesenchymal stem cells (MSCs) were engineered to express human sodium iodide symporter (NIS), a clinically relevant positron emission tomography (PET) reporter gene, as well as labeled with superparamagnetic iron oxide nanoparticles (SPIOs) to allow for detection with magnetic particle imaging (MPI). MSCs were additionally engineered with a preclinical bioluminescence imaging (BLI) reporter gene for comparison of BLI cell viability data to both MPI and PET data over time. MSCs were implanted into the hind limbs of immunocompromised mice and imaging with MPI, BLI and PET was performed over a 30-day period. MPI showed sensitive detection that steadily declined over the 30-day period, while BLI showed initial decreases followed by later rapid increases in signal. The PET signal of MSCs was significantly higher than the background at later timepoints. Early-phase imaging (day 0-9 post MSC injections) showed correlation between MPI and BLI data (R2 = 0.671), while PET and BLI showed strong correlation for late-phase (day 10-30 post MSC injections) imaging timepoints (R2 = 0.9817). We report the first use of combined MPI and PET for cell tracking and show the complementary benefits of MPI for sensitive detection of MSCs early after implantation and PET for longer-term measurements of cell viability.
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