寡核苷酸
DNA
复式(建筑)
化学
基因
计算生物学
细胞生物学
核酸
小RNA
DNA损伤
生物物理学
纳米技术
生物
生物化学
材料科学
作者
Jie Zeng,Liang Fang,Tingting Jiang,Mingchun Liu,Wei Li,Chengde Mao,Shifeng Lou,Hua Zuo
标识
DOI:10.1002/mabi.202200453
摘要
Antisense DNA oligonucleotide (AS) technology is a promising approach to regulate gene expression and cellular processes. For example, ASs can be used to capture the overexpressed, oncogenic miRNAs in tumors to suppress tumor growth. Among many challenges faced by AS approach is the degradation of ASs by nucleases under physiological conditions. Elongating the AS lifespan can substantially enhance the functions of AS. The paper reports a simple strategy to increase the stability of ASs. The authors discover that the ASs degrade quickly if their ends are in unpaired, single-stranded form, but much slower if their ends are in paired duplex form. It is conceivable to integrate this strategy with other strategies (such as chemical modification of ASs backbones) to maximally increase the ASs stabilities.
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